名稱(chēng)：毛細(xì)管色譜柱

固定相：6% 氰丙基苯基／94% 二甲基聚硅氧烷
性：弱性到中等性固定相
相當(dāng)于 USP 固定相 G43

型號(hào)：OV-624

規(guī)格：30m*0.32mm*1.8um
應(yīng)用： 毛細(xì)管氣相色譜法測(cè)定卡泊三醇中甲酸殘留量

滕州市浩瀚色譜儀器技術(shù)服務(wù)有限公司，建立卡泊三醇中甲酸的含量測(cè)定方法。方法使用氣相色譜儀,采用OV-624毛細(xì)管色譜柱(6%氰丙基苯聚硅氧烷-94%二甲基聚硅氧烷),0.32 mm×30 m,液膜厚度為1.8μm;柱溫40℃,保持5 min,以10℃.min-1的速度升溫150℃;保持10 min。頂空進(jìn)樣,頂空瓶平衡溫度:70℃,平衡時(shí)間:30 min,進(jìn)樣量為1 ml。以氮?dú)鉃檩d氣,流速為3 ml.min-1;FID檢測(cè)器。結(jié)果甲酸線性關(guān)系良好,相關(guān)系數(shù)大于0.999;平均回收率為100.1%;檢出限為1.19 mg.L-1。結(jié)論該方法簡(jiǎn)便,靈敏度高,重復(fù)性好,結(jié)果準(zhǔn)確,適合于卡泊三醇中甲酸殘留量的測(cè)定。

毛細(xì)管柱測(cè)卡泊三醇中甲酸殘留 測(cè)試譜圖：

Determination of formic acid residues in calcipotriol by capillary column
Determination of formic acid residues in calcipotriol by capillary column Details:

Name: Capillary chromatography column

Stationary phase: 6% cyanopropylphenyl/94% dimethyl polysiloxane
Polarity: weak polar to medium polar stationary phase
Equivalent to USP stationary phase G43

Model: OV-624

Specification: 30m*0.32mm*1.8um
Application: Determination of formic acid residue in calcipotriol by capillary gas chromatography

Tengzhou Haohan Chromatography Instrument Technology Service Co., Ltd. established a method for the determination of formic acid in calcipotriol. Methods Using a gas chromatograph, an OV-624 capillary chromatography column (6% cyanopropylbenzene polysiloxane-94% dimethyl polysiloxane), 0.32 mm × 30 m, liquid film thickness of 1.8 μm; column Keep the temperature at 40℃ for 5 minutes, and increase the temperature to 150℃ at the rate of 10℃.min-1; hold for 10 minutes. Headspace injection, headspace bottle equilibrium temperature: 70℃, equilibrium time: 30 min, injection volume is 1 ml. With nitrogen as the carrier gas, the flow rate is 3 ml.min-1; FID detector. Results The linear relationship of formic acid was good, the correlation coefficient was greater than 0.999; the average recovery was 100.1%; the detection limit was 1.19 mg.L-1. Conclusion The method is simple, sensitive, reproducible and accurate. It is suitable for the determination of formic acid residue in calcipotriol.

Capillary column measurement of formic acid residues in calcipotriol Test spectrum: